Reaction: TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O => thymidine or (deoxy)uridine + orthophosphate [NT5C]
- in pathway: Pyrimidine catabolism
Cytosolic 5'3'-nucleotidase (NT5C) catalyzes the hydrolysis of uridine 2', 3', and 5' monophosphates, dexoyuridine 3' and 5' monophosphates, and thymidine monophosphate to yield the corresponding (deoxy)nucleosides and orthophosphate. The active form of the enzyme is a homodimer, with an absolute requirement for Mg++ (Hoglund and Reichard 1990; Rampazzo et al. 2000). This enzyme appears to play a central role in the "substrate cycles" that regulate cytosolic deoxynucleotide levels (Gazziola et al. 2001).
Reaction - small molecule participants:
Pi [cytosol]
H2O [cytosol]
Reactome.org reaction link: R-HSA-109480
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Reaction input - small molecules:
water
Reaction output - small molecules:
hydrogenphosphate
Reactome.org link: R-HSA-109480