Reaction: Cleavage of formamidopyrimidine (FapyA) by NTHL1 glycosylase
- in pathway: Cleavage of the damaged pyrimidine
NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a FapyA DNA glycosylase to cleave FapyA (4,6-diamino-5-formamidopyrimidine), an imidazole ring-opened adenine derivative formed during oxidative stress (Evans et al. 2004), from the DNA sugar phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Luna et al. 2000, Hu et al. 2005). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).
Reaction - small molecule participants:
FapyA [nucleoplasm]
Reactome.org reaction link: R-HSA-110229
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Reaction input - small molecules:
Reaction output - small molecules:
4,6-diamino-5-formamidopyrimidine
Reactome.org link: R-HSA-110229