Reaction: Activation of ISG15 by UBA7 E1 ligase
- in pathway: ISG15 antiviral mechanism
Ubiquitin ligase UBE1L is the ISG15 activating enzyme. UBE1L activates ISG15 in an ATP-dependent process that links UBE1L to ISG15 via a thioester bond. Arginine 153 (R153) in human ISG15 is identified as the key residue for ISG15 and UBE1L's interaction.
Reaction - small molecule participants:
PPi [cytosol]
AMP [cytosol]
ATP [cytosol]
Reactome.org reaction link: R-HSA-1169397
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Reaction input - small molecules:
ATP(4-)
Reaction output - small molecules:
diphosphate(3-)
adenosine 5'-monophosphate(2-)
Reactome.org link: R-HSA-1169397