Reaction: phosphatidylinositol + UDP-N-acetyl-D-glucosamine -> N-acetylglucosaminyl-PI + UDP
- in pathway: Synthesis of glycosylphosphatidylinositol (GPI)
The first step of GPI synthesis is the transfer of N-acetylglucosamine from cytosolic UDP-N-acetylglucosamine to phosphatidyl inositol (PI) in the endoplasmic reticulum membrane. The reaction is catalyzed by a multimeric enzyme, also localized to the endoplasmic reticulum membrane, seven components of which have been identified to date by mutagenesis studies in cultured cells and by co-precipitation and reconstitution studies in vitro (Murakami et al. 2005; Watanabe et al. 1996, 1998, 2000).
Reaction - small molecule participants:
N-acetylglucosaminyl-PI [integral component of cytoplasmic side of endoplasmic reticulum membrane]
H+ [cytosol]
UDP [cytosol]
PI [endoplasmic reticulum membrane]
UDP-GlcNAc [cytosol]
Reactome.org reaction link: R-HSA-162730
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Reaction input - small molecules:
1-phosphatidyl-1D-myo-inositol(1-)
UDP-N-acetyl-alpha-D-glucosamine(2-)
Reaction output - small molecules:
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol(1-)
hydron
UDP(3-)
Reactome.org link: R-HSA-162730