Reaction: triacylglycerol + H2O -> diacylglycerol + fatty acid
- in pathway: Triglyceride catabolism
Activated rat HSL at the lipid particle hydrolyzes triacylglycerol to yield diacylglycerol + fatty acid. In vitro, activated partially purified HSL catalyzes this reaction at only about two times the rate measured with non-activated enzyme (Fredrikson et al. 1981). The much greater rate increase caused by HSL phosphorylation in vivo appears to be due to its phosphorylation-dependent translocation to the surface of the lipid particle (Birnbaum 2003).
HSL-mediated triacylglycerol hydrolysis in humans has not been studied in detail, so the human reaction is inferred from the well-studied rat one.
Reaction - small molecule participants:
LCFA(-) [cytosol]
DAG [lipid droplet]
H2O [cytosol]
TAG [lipid droplet]
Reactome.org reaction link: R-HSA-163551
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Reaction input - small molecules:
water
triglyceride
Reaction output - small molecules:
long-chain fatty acid anion
1,2-diacyl-sn-glycerol
Reactome.org link: R-HSA-163551