Reaction: Heparanase (HPSE) cleaves heparan sulfate from its proteoglycan (lysosome)
- in pathway: HS-GAG degradation
Heparanase (HPSE) is an endoglycosidase that cleaves heparan sulfate (HS) from its HS proteoglycan (HSPG) (Toyoshima & Nakajima 1999; Okada et al. 2002). The formation of a heterodimer of 8kDa and 50kDa subunits cleaved from the 65kDa form is required for enzyme activity (Levy-Adam et al. 2003) and this proteolytic cleavage occurs in the lysosome (Goldshmidt et al. 2002). Acidic conditions within the lysosome optimises HPSE activity.
Reaction - small molecule participants:
Heparan sulfate chain(1) [lysosomal lumen]
H2O [lysosomal lumen]
Reactome.org reaction link: R-HSA-1667005
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Reaction input - small molecules:
water
Reaction output - small molecules:
beta-D-IdoA-(1->4)-alpha-D-GlcNS6S-(1->4)-beta-D-IdoA2S-(1->4)-alpha-D-GlcNS3S-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl-yl group
Reactome.org link: R-HSA-1667005