Reaction: PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane
- in pathway: Synthesis of PIPs at the Golgi membrane
At the Golgi membrane, the PAS complex, consisting of FYVE finger-containing phosphoinositide kinase (PIKFYVE), yeast VAC14 homologue (VAC14), and polyphosphoinositide phosphatase aka SAC3 (FIG4), binds to the membrane via PIKFYVE's FYVE finger (Sbrissa et al. 2002). The PIKFYVE kinase component phosphorylates phosphatidylinositol 3-phosphate (PI3P) to phosphatidylinositol 3,5-bisphosphate PI(3,5)P2 (Sbrissa et al. 1999, McEwen et al. 1999). The PAS complex is present in the cytosol and is recruited to the membrane (Sbrissa et al. 2007). VAC14 acts as a scaffolding protein via its C-terminal domain (Sbrissa et al. 2008).
Reaction - small molecule participants:
ADP [cytosol]
PI(3,5)P2 [Golgi membrane]
ATP [cytosol]
PI3P [Golgi membrane]
Reactome.org reaction link: R-HSA-1675921
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Reaction input - small molecules:
ATP(4-)
1-phosphatidyl-1D-myo-inositol 3-phosphate(3-)
Reaction output - small molecules:
ADP(3-)
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate(5-)
Reactome.org link: R-HSA-1675921