Reaction: PI(3,4)P2 is dephosphorylated to PI4P by PTEN at the plasma membrane
- in pathway: Synthesis of PIPs at the plasma membrane
At the plasma membrane, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase aka phosphatase and tensin homolog (PTEN) dephosphorylates phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) to phosphatidylinositol 4-phosphate (PI4P) (Myers et al. 1998, Das et al. 2003).
Early studies indicated that magnesium ion, Mg2+, was needed for the catalytic activity of PTEN isolated from bovine thymus (Kabuyama et al. 1996). Subsequent studies have shown that PTEN was catalytically active in buffers free of magnesium and magnesium was not detected as part of the PTEN crystal (Lee et al. 1999).
Early studies indicated that magnesium ion, Mg2+, was needed for the catalytic activity of PTEN isolated from bovine thymus (Kabuyama et al. 1996). Subsequent studies have shown that PTEN was catalytically active in buffers free of magnesium and magnesium was not detected as part of the PTEN crystal (Lee et al. 1999).
Reaction - small molecule participants:
Pi [cytosol]
PI4P [plasma membrane]
H2O [cytosol]
PI(3,4)P2 [plasma membrane]
Reactome.org reaction link: R-HSA-1676149
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Reaction input - small molecules:
water
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
Reaction output - small molecules:
hydrogenphosphate
1-phosphatidyl-1D-myo-inositol 4-phosphate
Reactome.org link: R-HSA-1676149