Reaction: GSS:Mg2+ dimer synthesizes GSH
- in pathway: Glutathione synthesis and recycling
In the second step in the formation of glutathione, gamma-glutamylcysteine (gGluCys) ligates with glycine (Gly) to form glutathione (GSH) (Gali & Board 1995). This reaction is catalysed by glutathione synthetase (GSS), a homodimeric enzyme present in the cytosol which requires one Mg2+ cofactor per subunit for activity (Polekhina et al. 1999).
Reaction - small molecule participants:
Pi [cytosol]
ADP [cytosol]
GSH [cytosol]
gGluCys [cytosol]
ATP [cytosol]
Gly [cytosol]
Reactome.org reaction link: R-HSA-174394
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Reaction input - small molecules:
L-gamma-glutamyl-L-cysteinate(1-)
ATP(4-)
glycine zwitterion
Reaction output - small molecules:
hydrogenphosphate
ADP(3-)
glutathionate(1-)
Reactome.org link: R-HSA-174394