Reaction: Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere
- in pathway: Polymerase switching on the C-strand of the telomere
The binding of the primer recognition complex involves the loading of the proliferating cell nuclear antigen (PCNA). Replication Factor C (RFC) transiently opens the PCNA toroid in an ATP-dependent reaction, and then allows PCNA to re-close around the double helix adjacent to the primer terminus. This leads to the formation of the "sliding clamp" (Tsurimoto et al. 1990, Mossi and Hubscher 1998). In a human telomere replication model, RFC-mediated PCNA loading increases the processivity of telomeric C-strand synthesis, but does not eliminate polymerase delta stalling on the G-rich template (Lormand et al. 2013).
Interaction of RTEL1 with PCNA is needed for telomere replication and maintenance of telomere integrity (Vannier et al. 2013).
Interaction of RTEL1 with PCNA is needed for telomere replication and maintenance of telomere integrity (Vannier et al. 2013).
Reaction - small molecule participants:
Pi [nucleoplasm]
ADP [nucleoplasm]
H2O [nucleoplasm]
ATP [nucleoplasm]
Reactome.org reaction link: R-HSA-174439
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Reaction input - small molecules:
water
ATP(4-)
Reaction output - small molecules:
hydrogenphosphate
ADP(3-)
Reactome.org link: R-HSA-174439