Reaction: Cyclin E:CDK2-mediated phosphorylation of RB1
- in pathway: Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes
Cyclin E (CCNE1 or CCNE2) forms a complex with CDK2 and collaborates with the cyclin D-dependent kinases CDK4 and CDK6 in phosphorylating RB1 (Kelly et al.1998, Adams et al.1999). CDK4/CDK6-mediated monophosphorylation of RB1 is a pre-requisite for hyperphosphorylation and full inactivation of RB1 mediated by the CCNE:CDK2 complex (Connell-Crowley et al. 1997, Lundberg and Weinberg 1998, Brown et al. 1999, Harbour et al. 1999, Yu et al. 2000, Ezhevsky et al. 2001), with monophosphorylated RB1 being phosphorylated on 14 CDK sites out of 16 predicted sites (Narasimha et al. 2014).
Reaction - small molecule participants:
ADP [nucleoplasm]
ATP [nucleoplasm]
Reactome.org reaction link: R-HSA-188390
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Reaction input - small molecules:
ATP(4-)
Reaction output - small molecules:
ADP(3-)
Reactome.org link: R-HSA-188390