Reaction: 25(S) THCA-CoA is dehydrogenated to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA)
25(S) THCA-CoA and O2 react to form 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA) and H2O2. This dehydrogenation reaction occurs in the peroxisomal matrix. It is catalyzed by the FAD-containing peroxisomal enzyme branched chain acyl-CoA oxidase (ACOX2). The enzyme transfers electrons to molecular oxygen and hydrogen peroxide is produced as a byproduct.
Reaction - small molecule participants:
H2O2 [peroxisomal matrix]
THCA-CoA [peroxisomal matrix]
O2 [peroxisomal matrix]
25(S) THCA-CoA [peroxisomal matrix]
Reactome.org reaction link: R-HSA-192335
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Reaction input - small molecules:
dioxygen
(25S)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA
Reaction output - small molecules:
hydrogen peroxide
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-en-26-oyl-CoA
Reactome.org link: R-HSA-192335