Reaction: 3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestanoate (TetraHCA)
3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestan-27-al, NADPH + H+, and O2 react to form 3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestanoate (TetraHCA), NADP+, and H2O. This oxidation reaction occurs in the mitochondrial matrix, catalyzed by CYP27A1.
Reaction - small molecule participants:
NADP+ [mitochondrial matrix]
TetraHCA [mitochondrial matrix]
H2O [mitochondrial matrix]
O2 [mitochondrial matrix]
3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestan-27-al [mitochondrial matrix]
H+ [mitochondrial matrix]
NADPH [mitochondrial matrix]
Reactome.org reaction link: R-HSA-193713
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Reaction input - small molecules:
dioxygen
(24S)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-al
hydron
NADPH
Reaction output - small molecules:
NADP(+)
(24S)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oate
water
Reactome.org link: R-HSA-193713