Reaction: TetraHCA is conjugated with Coenzyme A (SLC27A2 VLCS)
TetraHCA (25(R) 3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestanoate), coenzyme A, and ATP react to form the CoA conjugate of 25(R) TetraHCA, AMP, pyrophosphate and water. This cytosolic reaction is catalyzed by SLC27A2 (VLCS). SLC27A5 (BACS) also catalyzes this reaction; the relative contributions of the two enzymes to de novo bile acid synthesis in vivo are not certain (Mihalik et al. 2002).
Reaction - small molecule participants:
PPi [cytosol]
25(R) TetraHCA-CoA [cytosol]
H2O [cytosol]
AMP [cytosol]
ATP [cytosol]
CoA-SH [cytosol]
TetraHCA [cytosol]
Reactome.org reaction link: R-HSA-193727
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Reaction input - small molecules:
ATP(4-)
coenzyme A(4-)
(24S)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oate
Reaction output - small molecules:
diphosphate(3-)
3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA
water
adenosine 5'-monophosphate(2-)
Reactome.org link: R-HSA-193727