Reaction: 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA)
3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al, NADPH + H+, and O2 react to form 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA), NADP+, and H2O. This oxidation reaction occurs in the mitochondrial matrix, catalyzed by CYP27A1.
Reaction - small molecule participants:
3,7,24THCA [mitochondrial matrix]
NADP+ [mitochondrial matrix]
H2O [mitochondrial matrix]
O2 [mitochondrial matrix]
H+ [mitochondrial matrix]
3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al [mitochondrial matrix]
NADPH [mitochondrial matrix]
Reactome.org reaction link: R-HSA-193737
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Reaction input - small molecules:
dioxygen
hydron
(24S)-3alpha,7alpha,24-trihydroxy-5beta-cholestan-26-al
NADPH
Reaction output - small molecules:
3alpha,7alpha,24-trihydroxy-5beta-cholestan-26-oic acid
NADP(+)
water
Reactome.org link: R-HSA-193737