Reaction: 3,7,24THCA is conjugated with Coenzyme A (SLC27A2 VLCS)
3,7,24THCA (25(R) 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate), coenzyme A, and ATP react to form the CoA conjugate of 3,7,24THCA, AMP, pyrophosphate and water. This cytosolic reaction is catalyzed by SLC27A2 (VLCS). SLC27A5 (BACS) also catalyzes this reaction; the relative contributions of the two enzymes to de novo bile acid synthesis in vivo are not certain (Mihalik et al. 2002).
Reaction - small molecule participants:
PPi [cytosol]
H2O [cytosol]
3,7,24THCA-CoA [cytosol]
AMP [cytosol]
ATP [cytosol]
CoA-SH [cytosol]
3,7,24THCA [cytosol]
Reactome.org reaction link: R-HSA-193743
======
Reaction input - small molecules:
ATP(4-)
coenzyme A(4-)
3alpha,7alpha,24-trihydroxy-5beta-cholestan-26-oic acid
Reaction output - small molecules:
diphosphate(3-)
water
3alpha,7alpha,24-trihydroxy-5beta-cholestan-26-oyl-CoA
adenosine 5'-monophosphate(2-)
Reactome.org link: R-HSA-193743