Reaction: CHPF,CHSY3 transfer GalNAc to chondroitin
- in pathway: Chondroitin sulfate biosynthesis
An N-acetylgalactosamine (GalNAc) moiety is added to the chondroitin chain by dual-activity enzymes, the chondroitin sulfate synthases 1-3 (CHSY1, CHPF and CHSY3 respectively) (Kitagawa et al. 2001, Yada et al. 2003, Yada et al. 2003b). They possess both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity, the latter activity used in this reaction. These three enzymes require divalent metals as cofactors, manganese producing the highest activities. The repeated disaccharide units of GlcA-GalNAc identify this glycosaminoglycan as chondroitin.
Reaction - small molecule participants:
UDP [Golgi lumen]
UDP-GalNAc [Golgi lumen]
Reactome.org reaction link: R-HSA-1971487
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Reaction input - small molecules:
UDP-N-acetyl-D-galactosamine(2-)
Reaction output - small molecules:
UDP(3-)
Reactome.org link: R-HSA-1971487