Reaction: NADSYN1 hexamer amidates NAAD to NAD+
- in pathway: Nicotinate metabolism
NAD synthases 1 and 2 (NADSYN1 and NADSYN2) catalyse the final step in the biosynthesis of NAD+, both in de novo synthesis and in the salvage pathway. The enzymes makes use of an amide donor in the reaction. NADSYNs exist as a homohexamers in the cytosol. The major difference between the two forms is that NADSYN1 appears to be glutamine-dependent whereas NADSYN2 is strictly ammonia-dependent (Hara et al. 2003).
Reaction - small molecule participants:
PPi [cytosol]
L-Glu [cytosol]
AMP [cytosol]
NAD+ [cytosol]
H2O [cytosol]
NAAD [cytosol]
ATP [cytosol]
L-Gln [cytosol]
Reactome.org reaction link: R-HSA-197271
======
Reaction input - small molecules:
water
deamido-NAD(+)
ATP(4-)
L-glutamine zwitterion
Reaction output - small molecules:
diphosphate(3-)
L-glutamate(1-)
adenosine 5'-monophosphate(2-)
NAD(1-)
Reactome.org link: R-HSA-197271