Reaction: DHPS tetramer synthesizes Dhp-K50-EIF5A from EIF5A and spermidine
- in pathway: Hypusine synthesis from eIF5A-lysine
Cytosolic deoxyhypusine synthase (DHPS) tetramer catalyzes the reaction of of EIF5A protein, spermidine (SPM), and NAD+ to convert lysine 50 of EIF5A to deoxyhypusine (Dhp), generating 1,3 diaminopropane, NADH and H+ in the process (Clement et al. 2003; Joe et al. 1995; Park 2006; Wolff et al. 1997). Although the reaction is reversible, the reverse reaction is probably minimized under physiological conditions by the rapid, irreversible conversion of EIF5A Dhp residues to hypusine.
Reaction - small molecule participants:
H+ [cytosol]
1,3-diaminopropane [cytosol]
NADH [cytosol]
SPM [cytosol]
NAD+ [cytosol]
Reactome.org reaction link: R-HSA-204647
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Reaction input - small molecules:
spermidine(3+)
NAD(1-)
Reaction output - small molecules:
hydron
trimethylenediamine
NADH(2-)
Reactome.org link: R-HSA-204647