Reaction: DOHH:Fe2+ hydroxylates Dhp-K50-EIF5A to form Hyp-K50-EIF5A
- in pathway: Hypusine synthesis from eIF5A-lysine
Cytosolic deoxyhypusine hydroxylase (DOHH) complexed with Fe2+ catalyzes the irreversible hydroxylation of peptidyl deoxyhypusine (Dhp-K50-EIF5A) to peptidyl hypusine (Hyp-K50-EIF5A) using molecular oxygen. The only known substrate for this enzyme is the modified lysine at residue 50 of the two isoforms of eIF5A (Clement et al. 2003; Kang et al. 2007; Kim et al. 2006).
Reaction - small molecule participants:
H2O [cytosol]
NAD+ [cytosol]
H+ [cytosol]
O2 [cytosol]
NADH [cytosol]
Reactome.org reaction link: R-HSA-204662
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Reaction input - small molecules:
hydron
dioxygen
NADH(2-)
Reaction output - small molecules:
water
NAD(1-)
Reactome.org link: R-HSA-204662