Meta-Biol

• Pathways

Cytosolic PCK1 (phosphoenolpyruvate carboxykinase 1) catalyzes the reaction of carbovir diphosphate and ATP to form carbovir triphosphate and ADP. The activity of human PCK1 and relative inactivity of human nucleoside diphosphate kinase are inferred from the properties of the purified rat and bovine enzymes in vitro (Miller et al. 1992). Carbovir triphosphate is the active metabolite that inhibits HIV infection by acting both as a competitive inhibitor of the natural deoxynucleoside triphosphates at the reverse transcriptase and as a DNA chain terminator following incorporation of the inhibitor into viral DNA (Faletto et al. 1997).