Reaction: RPE65 isomero-hydrolyses atREs to 11cROL
- in pathway: The canonical retinoid cycle in rods (twilight vision)
All-trans-retinol esters (atREs) serve as substrates for retinoid isomerohydrolase (RPE65), located in retinal pigment epithelium (RPE) cells. RPE65 hydrolyses atREs to 11-cis-retinol (11cROL), thus performing an isomerase activity as well as hydrolysis. RPE65 is membrane-bound, this being dependent on the palmitylation of the residue Cys-112 (Takahashi et al. 2009). RPE65 normally undergoes a light-dependent translocation to become more concentrated in the central region of RPE cells. This translocation requires Unconventional myosin-VIIa (MYO7A or USH1B) (Lopes et al. 2011).
Reaction - small molecule participants:
11cROL [cytosol]
H+ [cytosol]
H2O [cytosol]
Reactome.org reaction link: R-HSA-2453833
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Reaction input - small molecules:
water
Reaction output - small molecules:
11-cis-retinol
hydron
Reactome.org link: R-HSA-2453833