Reaction: alanine + tRNA(Ala) + ATP => Ala-tRNA(Ala) + AMP + pyrophosphate
- in pathway: Cytosolic tRNA aminoacylation
AARS (cytosolic alanyl tRNA synthetase) catalyzes the reaction of alanine, tRNA(ala), and ATP to form Ala tRNA(Ala), AMP, and pyrophosphate. The enzyme, a class II tRNA synthetase, is a monomer (Shiba et al. 1995). A mutation in the editing domain of the mouse Aars gene results in misincorporation of non-cognate amino acids into cellular proteins. This is associated with protein misfolding in Purkinje cells and a phenotype consistent with human ataxia (Lee et al. 2006).
Reaction - small molecule participants:
PPi [cytosol]
AMP [cytosol]
ATP [cytosol]
L-Ala [cytosol]
Reactome.org reaction link: R-HSA-379864
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Reaction input - small molecules:
ATP(4-)
L-alanine zwitterion
Reaction output - small molecules:
diphosphate(3-)
adenosine 5'-monophosphate(2-)
Reactome.org link: R-HSA-379864