Reaction: isoleucine + tRNA(Ile) + ATP => Ile-tRNA(Ile) + AMP + pyrophosphate
- in pathway: Cytosolic tRNA aminoacylation
IARS (cytosolic aspartyl tRNA synthetase) catalyzes the reaction of isoleucine, tRNA(Ile), and ATP to form Ile-tRNA(Ile), AMP, and pyrophosphate. The enzyme, a class I tRNA synthetase, is found in the cell as a component of the mutienzyme aminoacyl-tRNA synthetase complex (Shiba et al. 1994).
Reaction - small molecule participants:
PPi [cytosol]
AMP [cytosol]
ATP [cytosol]
L-Ile [cytosol]
Reactome.org reaction link: R-HSA-379893
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Reaction input - small molecules:
ATP(4-)
L-isoleucine zwitterion
Reaction output - small molecules:
diphosphate(3-)
adenosine 5'-monophosphate(2-)
Reactome.org link: R-HSA-379893