Reaction: NLK phosphorylates TCF/LEF
- in pathway: Ca2+ pathway
Activated NLK phosphorylates TCF7L2 and LEF1 at serine and threonine residues that lie in the central region between the DNA-binding and beta-catenin interaction domains. Phosphorylation does not affect the ability of TCF/LEF to bind to beta-catenin, but the phosphorylated complex is not able to bind to target DNA or activate transcription as assessed by EMSA and reporter gene assays (Ishitani et al, 2003b).
Reaction - small molecule participants:
ADP [nucleoplasm]
ATP [nucleoplasm]
Reactome.org reaction link: R-HSA-4411383
======
Reaction input - small molecules:
ATP(4-)
Reaction output - small molecules:
ADP(3-)
Reactome.org link: R-HSA-4411383