Reaction: Addition of a third glucose to the N-glycan precursor by an ALG10 homologue
- in pathway: Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
The last glucose is added to the N-glycan precursor. This reaction occurs inside the ER lumen and uses Dol-P-Glc as the glucose donor. In yeast, this reaction is catalyzed by ALG10 (Burda P and Aebi M,1998); however, this gene is duplicated in primates (Ciccarelli FD et al, 2005; Table 1), leading to two homologues, ALG10A and ALG10B, and to date there is no clear evidence to say which of these two paralogues (or both) is responsible for catalyzing this reaction in humans. No Congenital Disorders of Glycosylations are known to be associated with either gene.
Reaction - small molecule participants:
DOLP [integral component of lumenal side of endoplasmic reticulum membrane]
(Glc)3 (GlcNAc)2 (Man)9 (PP-Dol)1 [integral component of lumenal side of endoplasmic reticulum membrane]
DbGP [integral component of lumenal side of endoplasmic reticulum membrane]
(Glc)2 (GlcNAc)2 (Man)9 (PP-Dol)1 [integral component of lumenal side of endoplasmic reticulum membrane]
Reactome.org reaction link: R-HSA-446194
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Reaction input - small molecules:
dolichyl beta-D-glucosyl phosphate
glycan G10599
Reaction output - small molecules:
dolichyl phosphate(2-)
glycan G00008
Reactome.org link: R-HSA-446194