Reaction: ALG12 transfers Man to N-glycan precursor (GlcNAc)2 (Man)7 (PP-Dol)1
- in pathway: Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
The eighth mannose is added to the N-glycan precursor. This reaction occurs in the ER lumen and uses dolichyl phosphate D-mannose as a mannose donor. Defects in ALG12 are the cause of congenital disorder of glycosylation type 1G (CDG1G) (Chantret I et al, 2002).
Reaction - small molecule participants:
DOLP [integral component of lumenal side of endoplasmic reticulum membrane]
(GlcNAc)2 (Man)8 (PP-Dol)1 [integral component of lumenal side of endoplasmic reticulum membrane]
DOLP-Man [integral component of lumenal side of endoplasmic reticulum membrane]
(GlcNAc)2 (Man)7 (PP-Dol)1 [integral component of lumenal side of endoplasmic reticulum membrane]
Reactome.org reaction link: R-HSA-446198
======
Reaction input - small molecules:
dolichyl D-mannosyl phosphate(1-)
alpha-Man-(1->2)-alpha-Man-(1->2)-alpha-Man-(1->3)-[alpha-Man-(1->2)-alpha-Man-(1->3)-alpha-Man-(1->6)]-beta-Man-(1->4)-beta-GlcNAc-(1->4)-alpha-GlcNAc(PP-Dol)
Reaction output - small molecules:
dolichyl phosphate(2-)
alpha-Man-(1->2)-alpha-Man-(1->2)-alpha-Man-(1->3)-[alpha-Man-(1->6)-[alpha-Man-(1->2)-alpha-Man-(1->3)]-alpha-Man-(1->6)]-beta-Man-(1->4)-beta-GlcNAc-(1->4)-alpha-GlcNAc(PP-Dol)
Reactome.org link: R-HSA-446198