Reaction: ALG13:ALG14 transfers GlcNAc from UDP-GlcNAc to GlcNAcDOLP
- in pathway: Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
A second N-acetylglucosamine is added to the N-glycan precursor via a beta-1,4 linkage. This reaction is catalyzed by the ALG13:ALG14 complex, in which ALG13 functions as the catalyst and ALG14 functions as a membrane anchor which recruits ALG13 to the cytosolic face of ER (Gao XD et al, 2005).
Reaction - small molecule participants:
N,N'-DCDOLDP [integral component of cytoplasmic side of endoplasmic reticulum membrane]
UDP [cytosol]
UDP-GlcNAc [cytosol]
GlcNAcDOLDP [integral component of cytoplasmic side of endoplasmic reticulum membrane]
Reactome.org reaction link: R-HSA-446207
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Reaction input - small molecules:
UDP-N-acetyl-alpha-D-glucosamine(2-)
N-acetyl-D-glucosaminyldiphosphodolichol
Reaction output - small molecules:
diacetylchitobiosyldiphosphodolichol
UDP(3-)
Reactome.org link: R-HSA-446207