Reaction: alpha-methylacetoacetyl-CoA + NADH + H+ <=> alpha-methyl-beta-hydroxybutyryl-CoA + NAD+
- in pathway: Branched-chain amino acid catabolism
Mitochondrial 3-hydroxyacyl-CoA dehydrogenase type-2 (HSD17B10; HADH2) catalyzes the reversible reaction of alpha-methylacetoacetyl-CoA and NADH + H+ to form alpha-methyl-beta-hydroxybutyryl-CoA and NAD+ (Ofman et al. 2003). Crystallographic data indicate that the enzyme is a homotetramer (Kissinger et al. 2004).
Reaction - small molecule participants:
aMbHBUT-CoA [mitochondrial matrix]
NAD+ [mitochondrial matrix]
H+ [mitochondrial matrix]
2MACA-CoA [mitochondrial matrix]
NADH [mitochondrial matrix]
Reactome.org reaction link: R-HSA-508369
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Reaction input - small molecules:
hydron
2-methylacetoacetyl-CoA
NADH(2-)
Reaction output - small molecules:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA
NAD(1-)
Reactome.org link: R-HSA-508369