Reaction: TDG excises 5-formylcytosine
- in pathway: TET1,2,3 and TDG demethylate DNA
Thymine DNA glycosylase (TDG) excises 5-formylcytosine (5-fC) from DNA (Maiti and Drohat 2011, Zhang et al. 2012, inferred from mouse in He et al. 2011) by flipping the base out of the helix and cleaving the N-glycosidic bond to leave an abasic site (apurinic/apyrimidinic site, AP site). TDG interacts with the G opposite the excised base and remains bound to the abasic site (Maiti et al 2008). Dissociation of TDG from DNA is the rate-limiting step of the reaction.
Reaction - small molecule participants:
5-fC [nucleoplasm]
Reactome.org reaction link: R-HSA-5220959
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Reaction input - small molecules:
Reaction output - small molecules:
5-formylcytosine
Reactome.org link: R-HSA-5220959