Reaction: BLM mediates dissolution of double Holliday junction
The BTRR complex, composed of BLM, TOP3A, RMI1 and RMI2, dissolves double Holliday junctions by disentangling hemicatenane intermediates (Bocquet et al. 2014). This results in non-crossover products, where no exchange of genetic material happens between the sister chromatid that served as a template for the DNA repair synthesis and the repaired DNA duplex. SPIDR serves as a scaffold that connects the BTRR complex with the double Holliday junction through its simultaneous interaction with RAD51-coated DNA strands of the Holliday junction and BLM. SPIDR is needed for BTRR-mediated prevention of cross-over between sister chromatids (Wan et al. 2013).
Reaction - small molecule participants:
Pi [nucleoplasm]
ADP [nucleoplasm]
H2O [nucleoplasm]
ATP [nucleoplasm]
Reactome.org reaction link: R-HSA-5686410
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Reaction input - small molecules:
water
ATP(4-)
Reaction output - small molecules:
hydrogenphosphate
ADP(3-)
Reactome.org link: R-HSA-5686410