Reaction: Active RNA Pol II complex transcribes lesion-containing DNA template
- in pathway: Formation of TC-NER Pre-Incision Complex
An active RNA polymerase II complex (RNA Pol II, POLR2) transcribes a damaged DNA template. Once damaged DNA bases, such as cyclobutane pyrimidine dimers (CPDs), enter the active site of the polymerase, RNA Pol II misincorporates a ribonucleotide into nascent mRNA, which blocks the translocation step and results in polymerase stalling. In the stalled complex, the lesion is inaccessible, while the RNA Pol II conformation is unchanged (Brueckner et al. 2007).
Reaction - small molecule participants:
PPi [nucleoplasm]
ribonucleoside triphosphate [nucleoplasm]
Reactome.org reaction link: R-HSA-6781824
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Reaction input - small molecules:
ribonucleoside triphosphate
Reaction output - small molecules:
diphosphate(3-)
Reactome.org link: R-HSA-6781824