Reaction: HAGH hydrolyses (R)-S-LGSH to GSH and LACT
- in pathway: Pyruvate metabolism
In the second step of the glyoxalase system, hydroxyacylglutathione hydrolase (HAGH) catalyses the hydrolysis of (R)-S-lactoylglutathione ((R)-S-LGSH) to glutathione (GSH) and lactic acid (LACT) (Ridderstrom et al. 1996). The HAGH gene can produce two forms of the protein, form 1 is mitochondrial whereas form 2 is cytosolic (Cordell et al. 2004). HAGH is monomeric but requires two Zn2+ ions for activity (Cameron et al. 1999). This reaction completes the detoxification of methylglyoxal, a reactive byproduct of pyruvate metabolism.
Reaction - small molecule participants:
LACT [cytosol]
GSH [cytosol]
(R)-S-LGSH [cytosol]
Reactome.org reaction link: R-HSA-6783221
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Reaction input - small molecules:
(R)-S-lactoylglutathione
Reaction output - small molecules:
(S)-lactic acid
glutathionate(1-)
Reactome.org link: R-HSA-6783221