Reaction: MAN2C1 hydrolyses GlcNAc (Man)9 to GlcNAc (Man)5
- in pathway: Lysosomal oligosaccharide catabolism
Endoplasmic reticulum-associated degradation of misfolded glycoproteins ensures only functional, correctly folded proteins exit from the endoplasmic reticulum and that misfolded ones are targeted for lysosomal degradation. Misfolded glycoproteins are deglycosylated in the cytosol and the resultant free oligosaccharide can be further hydrolysed by alpha-mannosidase 2C1 (MAN2C1). For example, the oligosaccharide structure GlcNAc (Man)9 is hydrolysed by MAN2C1 to GlcNAc (Man)5, an intermediate structure formed in the N-glycan precursor biosynthesis pathway (Suzuki et al. 2006).
Reaction - small molecule participants:
Man [cytosol]
alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc [cytosol]
H2O [cytosol]
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc [cytosol]
Reactome.org reaction link: R-HSA-6799545
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Reaction input - small molecules:
water
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc
Reaction output - small molecules:
D-mannopyranose
alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc
Reactome.org link: R-HSA-6799545