Reaction: NT5E:Zn2+ hydrolyses NAD+
- in pathway: Nicotinate metabolism
5'-nucleotidase (NT5E, CD73) is able to hydrolyse extracellular nucleotides into membrane permeable nucleosides. It displays a broad specificity, acting on mono- or di-nucleotide nicotinamides and different adenosine phosphates, with maximal activity on 5'-adenosine monophosphate. Human NT5E can hydrolyse both NAD+ and NMN, suggesting a role in NAD metabolism (Garavaglia et al. 2012). NT5E is a glycolipid-anchored plasma membrane enzyme (Misumi et al. 1990) that is active in dimeric form and requires one zinc ion per subunit (Zimmermann 1992).
Reaction - small molecule participants:
Pi [extracellular region]
NMN [extracellular region]
NAD+ [extracellular region]
H2O [extracellular region]
Reactome.org reaction link: R-HSA-8940070
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Reaction input - small molecules:
NAD(1-)
water
Reaction output - small molecules:
hydrogenphosphate
NMN(-)
Reactome.org link: R-HSA-8940070