Meta-Biol

• Pathways

The following GTPase activating proteins (GAPs) were shown to bind RAC1 and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RAC1:GTP complex into the inactive RAC1:GDP complex (the high throughput screen by Bagci et al. 2020 examined binding of GAPs to constitutively active RAC1 mutant without testing for activation of RAC1 GTPase activity and is cited as supporting evidence, as indicated):
ARHGAP4 (Vogt et al. 2007; Müller et al. 2020)
ARHGAP9 (Furukawa et al. 2001; Müller et al. 2020)
ARHGAP15 (Seoh et al. 2003; Zamboni et al. 2016; Müller et al. 2020)
ARHGAP20 (Müller et al. 2020)
ARHGAP22 (Mori et al. 2014; Müller et al. 2020)
ARHGAP23 (Martin Vilchez et al. 2017; Müller et al. 2020)
ARHGAP27 (Sakakibara et al. 2004; Müller et al. 2020)
ARHGAP30 (Naji et al. 2011; Müller et al. 2020)
ARHGAP31 (Tcherkezian et al. 2006; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGAP39 (Lundström et al. 2004; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGAP44 (Raynaud et al. 2014; Müller et al. 2020)
CHN1 (Ahmed et al. 1994; Caloca et al. 2008; Müller et al. 2020)
CHN2 (Canagarajah et al. 2004; Caloca et al. 2003; Müller et al. 2020)
FAM13A (Müller et al. 2020)
FAM13B (Müller et al. 2020)
HMHA1 (de Kreuk et al. 2013)
RALBP1 (Jullien Flores et al. 1995; Müller et al. 2020)
SH3BP1 (Müller et al. 2020)
SRGAP2 (Mason et al. 2011; Guo and Bao 2010; Müller et al. 2020; supported by Bagci et al. 2020)
SRGAP3 (Endris et al. 2002; Müller et al. 2020)
SYDE2 (Müller et al. 2020)
TAGAP (Bauer et al. 2005; Müller et al. 2020)

The following GAPs were shown to bind RAC1 and stimulate its GTPase activity in some but not all studies and are annotated as candidate RAC1 GAPs (the high throughput screen by Bagci et al. 2020 examined binding of GAPs to constitutively active RAC1 mutant without testing for activation of RAC1 GTPase activity and is cited as supporting evidence, as indicated):
ABR (Chuang et al. 1995, Um et al. 2014, Amin et al. 2016; Müller et al. 2020: RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARAP1 (Müller et al. 2020: RAC1-directed GAP activity; Miura et al. 2002: no RAC1-directed GAP activity)
ARAP2 (Müller et al. 2020: RAC1 directed GAP activity; Bagci et al. 2020: binding to active RAC1; Yoon et al. 2006: no RAC1 directed GAP activity)
ARAP3 (Krugmann et al. 2002, Müller et al. 2020: RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARHGAP1 (Amin et al. 2016: RAC1 directed GAP activity; Li et al. 2009, Yang et al. 2006, Müller et al. 2020: no RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARHGAP5 (Burbelo et al. 1995: RAC1-directed GAP activity; Bagci et al. 2020: binding to active RAC1; Müller et al. 2020: no RAC1-directed GAP activity)
ARHGAP10 (Shibata et al. 2001: RAC1-directed GAP activity; Müller et al. 2020: no RAC1-directed GAP activity)
ARHGAP12 (Müller et al. 2020: RAC1-directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARHGAP17 (Richnau and Aspenstrom 2001, Amin et al. 2016: RAC1 directed GAP activity; Müller et al. 2020: no RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARHGAP21 (Bagci et al. 2020: binding to active RAC1; Sousa et al. 2005, Müller et al. 2020: no RAC1 directed GAP activity)
ARHGAP24 (Lavelin and Geiger 2005, Ohta et al. 2006: RAC1 directed GAP activity; Su et al. 2004, Müller et al. 2020: no RAC1 directed GAP activity)
ARHGAP25 (Csépányi Kömi et al. 2016, Csépányi Kömi et al. 2012: RAC1-directed GTPase activity; Müller et al. 2020: no RAC1-directed GAP activity)
ARHGAP26 (Amin et al. 2016: RAC1 directed GAP activity; Sheffield et al. 1999, Müller et al. 2020: no RAC1 directed GAP activity)
ARHGAP29 (Saras et al. 1997: RAC1 directed GAP activity; Müller et al. 2020: no RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARHGAP32 (Nakazawa et al. 2003: RAC1-directed GAP activity; Bagci et al. 2020: binding to active RAC1; Müller et al. 2020: no RAC1-directed GAP activity)
ARHGAP33 (Müller et al. 2020: RAC1-directed GAP activity; Liu et al. 2006: no RAC1-directed GAP activity)
ARHGAP35 (Lévay et al. 2009, Amin et al. 2016, Müller et al. 2020: RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
ARHGAP42 (Bagci et al. 2020: binding to active RAC1; Bai et al. 2013: no RAC1 directed GAP activity)
BCR (Um et al. 2014, Chuang et al. 1995, Müller et al. 2020: RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
DEPDC1B (Wu et al. 2015: RAC1-directed GAP activity; Bagci et al. 2020: binding to active RAC1; Müller et al. 2020: no RAC1-directed GAP activity)
DLC1 (Amin et al. 2016: RAC1 directed GAP activity; Homma and Emori 1995, Müller et al. 2020: no RAC1 directed GAP activity)
GMIP (Müller et al. 2020: RAC1-directed GAP activity; Aresta et al. 2002: no RAC1-directed GAP activity)
MYO9B (Müller et al. 2020: RAC1-directed GAP activity; Post et al. 1998, Kong et al. 2015: no RAC1-directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
OPHN1 (Niermann et al. 2016, Billuart et al. 1998, Elvers et al. 2012, Amin et al. 2016: RAC1 directed GAP activity; Müller et al. 2020: no RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
PIK3R1 (Bagci et al. 2020: binding to active RAC1; Müller et al. 2020: no RAC1-directed GAP activity)
PIK3R2 (Bagci et al. 2020: binding to active RAC1; Müller et al. 2020: no RAC1-directed GAP activity)
RACGAP1 (Touré et al. 1998, Amin et al. 2016, Müller et al. 2020: RAC1 directed GAP activity; Bagci et al. 2020: no binding to active RAC1)
SRGAP1 (Müller et al. 2020: RAC1-directed GAP activity; Wong et al. 2001: no RAC1-directed GAP activity)

The following GAPs do not act on RAC1 or were shown by Bagci et al. 2020 to not bind constitutively active RAC1 mutant, as indicated:
ARHGAP6 (Prakash et al. 2000; Müller et al. 2020)
ARHGAP8 (Lua and Low 2004; Müller et al. 2020)
ARHGAP11A (Lawson et al. 2016; Müller et al. 2020)
ARHGAP11B (Florio et al. 2015; Müller et al. 2020)
ARHGAP18 (Maeda et al. 2011; Müller et al. 2020)
ARHGAP19 (David et al. 2014; Müller et al. 2020)
ARHGAP28 (Yeung et al. 2014; Müller et al. 2020)
ARHGAP36 (Rack et al. 2014; Müller et al. 2020)
ARHGAP40 (Müller et al. 2020)
ARHGAP45 (Müller et al. 2020)
DEPDC1 (Müller et al. 2020)
INPP5B (Müller et al. 2020)
MYO9A (Müller et al. 2020; Bagci et al. 2020: no binding to active RAC1)
OCRL (Erdmann et al. 2007; Lichter Konecki et al. 2006; Müller et al. 2020; Bagci et al. 2020: no binding to active RAC1)
STARD8 (Kawai et al. 2007; Amin et al. 2016; Müller et al. 2020)
STARD13 (Ching et al. 2003; Amin et al. 2016; Müller et al. 2020)
SYDE1 (Müller et al. 2020; Bagci et al. 2020: no binding to active RAC1)