Reaction: USP9X hydrolyzes Ub:PEX5L yielding PEX5L and Ubiquitin
- in pathway: Peroxisomal protein import
The deubiquitinating enzyme USP9X hydrolyzes the thioester bond between the carboxyl terminus of ubiquitin and cysteine-11 of PEX5L (Grou et al. 2012). The thioester bond is unstable and appears to be also spontaneously (non-enzymatically) disrupted by nucleophilic attack of small metabolites such as reduced glutathione (Grou et al. 2009).
Reaction - small molecule participants:
H2O [cytosol]
Reactome.org reaction link: R-HSA-9033491
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Reaction input - small molecules:
water
Reaction output - small molecules:
Reactome.org link: R-HSA-9033491