Meta-Biol

• Pathways

A tail-anchored protein bound to ASNA1 (TRC40) (Stefanovic and Hegde 2007, Favaloro et al. 2008, Favaloro et al. 2010) docked at the WRB:CAMLG complex (also known as the WRB:CAML complex) located in the endoplasmic reticulum membrane is released into the ER membrane in an incompletely characterized reaction (Vilardi et al. 2011, Yamamoto and Sakisaka 2012, Vilardi et al. 2014, Colombo et al. 2016, Pfaff et al. 2016, Vogl et al. 2016). Experiments with yeast homologs (GET1 is the homologue of WRB and GET2 is the homologue of CAMLG) indicate that docking of ASNA1:ADP with WRB:CAMLG results in a conformational change in ASNA1 that causes release of the tail-anchored protein to WRB:CAMLG and then into the membrane (inferred from yeast homologs), ADP dissociates from ASNA1 at some point during the reaction. Exchange of ADP for ATP by ASNA1 then recycles ASNA1 back into the cytosol (inferred from yeast homologs).