Reaction: nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA
- in pathway: Replication of the SARS-CoV-1 genome
nsp14 acts as 3'-5' exonuclease (Minskaia et al. 2006, Chen et al. 2007) that preferentially excises mismatched nucleotides from double stranded RNA (Minskaia et al. 2006, Bouvet et al. 2012). Binding to nsp10 increases the exonuclease activity of nsp14 (Bouvet et al. 2012, Subissi et al. 2014, Bouvet et al. 2014). nsp14 increases the fidelity of human SARS coronavirus 1 (SARS-CoV-1) replication by the nsp12 RNA-dependent RNA polymerase by 21-fold (Eckerle et al. 2010).
Reaction - small molecule participants:
NMP [cytosol]
H2O [cytosol]
Reactome.org reaction link: R-HSA-9682603
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Reaction input - small molecules:
water
Reaction output - small molecules:
ribonucleoside monophosphate
Reactome.org link: R-HSA-9682603