Reaction: NUDT1 hydrolyzes O6-methyl-dGTP to O6-methyl-dGMP
- in pathway: Phosphate bond hydrolysis by NUDT proteins
NUDT1 (MTH1) catalyzes the reaction of O6-methyl-dGTP and water to form O6-methyl-dGMP and PPi (pyrophosphate) (Jemth et al. 2018). Four NUDT1 proteins have been identified, encoded by a single gene with alternative start codons (Oda et al. 1999). The shortest of these, NUDT1 p18, has been shown to be enzymatically active as a monomer associated with a magnesium ion (Mishima et al. 2004; Sakumi et al. 1993; Takagi et al. 2012). The longer isoforms all consist of the p18 polypeptide with aminoterminal extensions and are presumed to be active as well but have not been experimentally characterized. The p18 isoform is predominantly cytosolic (Kang et al. 1995).
Reaction - small molecule participants:
PPi [cytosol]
H+ [cytosol]
O(6)-methyl-dGMP(2-) [cytosol]
H2O [cytosol]
O(6)-methyl-dGTP(4-) [cytosol]
Reactome.org reaction link: R-HSA-9731590
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Reaction input - small molecules:
water
O(6)-methyl-dGTP(4-)
Reaction output - small molecules:
diphosphate(3-)
hydron
O(6)-methyl-dGMP(2-)
Reactome.org link: R-HSA-9731590