Reaction: Transfer of Ub from E2 to substrate and release of E2
Interaction of E3 with both substrate and E2-Ub, brings them into proximity so that ubiquitin is transferred from E2 to the substrate. In most cases the transfer of ubiquitin is direct from E2 to substrate, but in a small subset of E3s, it occurs via a covalent E3-Ub thioester intermediate (Raymond et al. 2009).
Reaction - small molecule participants:
PPi [cytosol]
AMP [cytosol]
ATP [cytosol]
Reactome.org reaction link: R-HSA-983140
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Reaction input - small molecules:
ATP(4-)
Reaction output - small molecules:
diphosphate(3-)
adenosine 5'-monophosphate(2-)
Reactome.org link: R-HSA-983140