Reaction: 27-hydroxycholesterol is 7alpha-hydroxylated
27-hydroxycholesterol, NADPH + H+, and O2 react to form cholest-5-ene-3beta,7alpha,27-triol, H2O, and NADP+. This reaction is catalyzed by CYP7B1 in the endoplasmic reticulum membrane. Defects in CYB7B1 are associated with failure of 7alpha-hydroxylation in vivo, and with liver damage, confirming both the function of the enzyme and the central role of the liver in this metabolic process (Setchell et al. 1998).
Reaction - small molecule participants:
H2O [cytosol]
NADP+ [cytosol]
Cholest-5-ene-3beta,7alpha,27-triol [endoplasmic reticulum membrane]
H+ [cytosol]
27HCHOL [endoplasmic reticulum membrane]
O2 [cytosol]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-191972
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Reaction input - small molecules:
hydron
26-hydroxycholesterol
dioxygen
NADPH(4-)
Reaction output - small molecules:
water
NADP(3-)
7alpha,26-dihydroxycholesterol
Reactome.org link: R-HSA-191972