Reaction: CYP7A1 7-hydroxylates CHOL
- in pathway: Endogenous sterols
Cholesterol, NADPH + H+, and O2 form 7alpha-cholesterol (5-cholesten-3beta, 7alpha-diol), NADP+,and H2O, in a reaction catalysed by CYP7A1 (cholesterol 7alpha-hydroylase) in the endoplasmic reticulum membrane. In the body, this enzyme is expressed only in liver, and its expression is tightly regulated at the level of transcription to determine the overall rate of bile acid and bile salt production (Noshiro et al. 1990).
Reaction - small molecule participants:
H2O [cytosol]
NADP+ [cytosol]
7 alpha-hydroxycholesterol [endoplasmic reticulum membrane]
H+ [cytosol]
CHOL [endoplasmic reticulum membrane]
O2 [cytosol]
NADPH [cytosol]
H2O [cytosol]
NADP+ [cytosol]
7 alpha-hydroxycholesterol [endoplasmic reticulum membrane]
H+ [cytosol]
CHOL [endoplasmic reticulum membrane]
O2 [cytosol]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-192051
======
Reaction input - small molecules:
hydron
cholesterol
dioxygen
NADPH(4-)
hydron
cholesterol
dioxygen
NADPH(4-)
Reaction output - small molecules:
water
NADP(3-)
7alpha-hydroxycholesterol
water
NADP(3-)
7alpha-hydroxycholesterol
Reactome.org link: R-HSA-192051