Reaction: CYP46A1 24-hydroxylates CHOL

- in pathway: Endogenous sterols
Cholesterol 24-hydroxylase (CYP46A1), an enzyme associated with the ER membrane, catalyses the 24-hydroxylation of cholesterol (CHOL) to 24-hydroxycholesterol (24OH-CHOL). In the body, this enzyme is expressed predominantly in the brain and is thought to play a major role in cholesterol turnover there (Lund et al. 1999, Mast et al. 2003).
Reaction - small molecule participants:
24OH-CHOL [endoplasmic reticulum membrane]
H2O [cytosol]
NADP+ [cytosol]
H+ [cytosol]
CHOL [endoplasmic reticulum membrane]
O2 [cytosol]
NADPH [cytosol]
24OH-CHOL [endoplasmic reticulum membrane]
H2O [cytosol]
NADP+ [cytosol]
H+ [cytosol]
CHOL [endoplasmic reticulum membrane]
O2 [cytosol]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-192061

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Reaction input - small molecules:
hydron
ChEBI:15378
cholesterol
ChEBI:16113
dioxygen
ChEBI:15379
NADPH(4-)
ChEBI:57783
hydron
ChEBI:15378
cholesterol
ChEBI:16113
dioxygen
ChEBI:15379
NADPH(4-)
ChEBI:57783
Reaction output - small molecules:
(24S)-24-hydroxycholesterol
ChEBI:34310
water
ChEBI:15377
NADP(3-)
ChEBI:58349
(24S)-24-hydroxycholesterol
ChEBI:34310
water
ChEBI:15377
NADP(3-)
ChEBI:58349
Reactome.org link: R-HSA-192061