Reaction: Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol

- in pathway: Pregnenolone biosynthesis
22beta-hydroxycholesterol, NADPH + H+, and O2 react to form 20alpha,22beta-hydroxycholesterol, NADP+ and H2O, catalyzed by CYP11A (P450scc) associated with the inner mitochondrial membrane.
Reaction - small molecule participants:
20a,22b-DHCHOL [mitochondrial inner membrane]
NADP+ [mitochondrial matrix]
H2O [mitochondrial matrix]
O2 [mitochondrial matrix]
22b-HCHOL [mitochondrial inner membrane]
H+ [mitochondrial matrix]
NADPH [mitochondrial matrix]
Reactome.org reaction link: R-HSA-193065

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Reaction input - small molecules:
dioxygen
ChEBI:15379
(22S)-22-hydroxycholesterol
ChEBI:1301
hydron
ChEBI:15378
NADPH
ChEBI:16474
Reaction output - small molecules:
(20R,22R)-20,22-dihydroxycholesterol
ChEBI:1294
NADP(+)
ChEBI:18009
water
ChEBI:15377
Reactome.org link: R-HSA-193065