Reaction: glutamate + ATP + NADPH + H+ => L-glutamate gamma-semialdehyde + NADP+ + ADP + orthophosphate [P5CS]
- in pathway: Glutamate and glutamine metabolism
Delta-1-pyrroline-5-carboxylate synthetase associated with the inner mitochondrial membrane catalyzes the two-step reaction that converts glutamate, ATP, and NADPH + H+ to L-glutamate gamma-semialdehyde, NADP+, ADP, and orthophosphate. Two P5CS isoforms have been identified; both are active when expressed in enzyme-deficient cells in culture (Hu et al. 1999). Unpublished crystallographic data (PDB 2H5G) indicate that the enzyme is a dimer.
Reaction - small molecule participants:
Pi [mitochondrial matrix]
ADP [mitochondrial matrix]
L-Glu5S [mitochondrial matrix]
NADP+ [mitochondrial matrix]
H+ [mitochondrial matrix]
ATP [mitochondrial matrix]
NADPH [mitochondrial matrix]
L-Glu [mitochondrial matrix]
Reactome.org reaction link: R-HSA-508040
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Reaction input - small molecules:
hydron
ATP(4-)
NADPH
L-glutamate(1-)
Reaction output - small molecules:
hydrogenphosphate
ADP(3-)
L-glutamic 5-semialdehyde zwitterion
NADP(+)
Reactome.org link: R-HSA-508040