Reaction: CYP3A4,5 hydroxylates AFB1 to AFQ1
- in pathway: Aflatoxin activation and detoxification
Metabolites that are formed from aflatoxin B1 (AFB1) include AFQ1, AFM1 and AFP1 (Gallagher et al. 1996). These metabolites and other naturally occurring aflatoxins (G1, B2 and G2) are poorer substrates for epoxidation and, consequently, are less mutagenic, carcinogenic and toxic than AFB1. AFB1 metabolites can be useful biomarkers of human exposure to aflatoxins and AFM1, AFQ1 and AFP1 have all been detected in human urine samples (Groopman et al. 1985). Cytochrome P450 3A4 and 3A5 are the predominant enzymes involved in AFQ1 (3-hydroxy aflatoxin) production (Raney et al. 1992).
Reaction - small molecule participants:
AFQ1 [cytosol]
H2O [cytosol]
NADP+ [cytosol]
H+ [cytosol]
O2 [cytosol]
AFB1 [cytosol]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-5423664
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Reaction input - small molecules:
hydron
dioxygen
aflatoxin B1
NADPH(4-)
Reaction output - small molecules:
aflatoxin Q1
water
NADP(3-)
Reactome.org link: R-HSA-5423664