Reaction: MICAL1 produces NADP+, H2O2
MICAL1 is involved in cytoskeleton dynamics through its NADPH-dependent oxidase and F-actin depolymerizing activities. MICAL1 inhibits actin polymerization and promotes actin depolymerization (Hung et al. 2011). F-actin is thought to bind and stabilize the flavoprotein domain in MICAL's active conformation (Kolk & Pasterkamp 2007). Actin depolymerization mediated by MICAL1 is thought to be a consequence of H2O2 produced by MICAL1 NADPH oxidase activity (Nadella et al. 2005, Zucchini et al. 2011), rather than due to direct hydroxylation of actin methionine residues (Vitali et al. 2016).
Reaction - small molecule participants:
NADP+ [cytosol]
H2O2 [cytosol]
O2 [cytosol]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-8865107
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Reaction input - small molecules:
dioxygen
NADPH(4-)
Reaction output - small molecules:
NADP(3-)
hydrogen peroxide
Reactome.org link: R-HSA-8865107