Reaction: nsp12 transfers guanylyl onto SARS-CoV-2 plus strand subgenomic RNAs
- in pathway: Transcription of SARS-CoV-2 sgRNAs
Capping of viral mRNA starts with the transfer of a guanylyl residue onto the RNA`s phosphate moiety, creating the cap core structure (GpppA, also cap(-1)). SARS-CoV-2 Nsp12 has been shown to have this guanylyltransferase (GTase) activity, carrying out the addition of a GTP nucleotide to the 5' end of viral RNA, which can be inhibited by remdesivir triphosphate, the active form of the antiviral drug remdesivir (Yan et al, 2021; Walker et al, 2021).
Reaction - small molecule participants:
PPi [cytosol]
GTP [cytosol]
H+ [cytosol]
Reactome.org reaction link: R-HSA-9815529
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Reaction input - small molecules:
GTP(4-)
hydron
Reaction output - small molecules:
diphosphate(3-)
Reactome.org link: R-HSA-9815529