Reaction: Cholesterol is hydroxylated to 25-hydroxycholesterol
- in pathway: Synthesis of bile acids and bile salts
The microsomal enzyme cholesterol 25-hydroxylase is a member of a lipid metabolizing enzyme family that utilizes oxygen and diiron-oxygen cofactor to hydroxylate, desaturate, epoxidate and acetylate substrates.
Reaction - small molecule participants:
H2O [cytosol]
25OH-CHOL [cytosol]
NADP+ [cytosol]
CHOL [cytosol]
H+ [cytosol]
O2 [cytosol]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-191983
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Reaction input - small molecules:
cholesterol
hydron
dioxygen
NADPH(4-)
Reaction output - small molecules:
water
25-hydroxycholesterol
NADP(3-)
Reactome.org link: R-HSA-191983